FUNCTIONAL ANALYSIS OF MEDICINAL PLANTS USING SYSTEMS BIOLOGY APPROACHES

Plant derived medicine is an important source of life saving drugs, but the genome information of most important medicinal plants is still unavailable. The need of the hour is to identify more functional genes and enzymes that control secondary metabolite production in medical plants, develop new methods for systematics, engineer resistance to number of biotic and abiotic stresses, and develop new conservation strategies, more genomics, proteomics and metabolomics information needs to be produced. In this review, a brief overview of various omic technologies and its applications to medicinal and aromatic plants are discussed.Â

Physiological and biochemical responses involved in vegetative desiccation tolerance of resurrection plant Selaginella brachystachya

International audienceThe vegetative desiccation tolerance of Selaginella brachystachya has been evaluated for its ability to revive from a desiccation (air dry) state and start normal functioning when rehydrated. In this study, S. brachystachya was identified by DNA barcoding. Experiments were conducted using the detached hydrated, desiccated and rehydrated fronds under laboratory conditions to understand the mechanism of revival upon the water availability. Scanning Electron Microscope images during desiccation showed closed stomata and inside curled leaves. Chlorophyll concentration decreased by 1.1 fold in desiccated state and recovered completely upon rehydration. However, the total carotenoid content decreased 4.5 fold while the anthocyanin concentration increased 5.98 fold and the CO2 exchange rate became negative during desiccation. Lipid peroxidation and superoxide radical production were enhanced during desiccation by 68.32 and 73.4%, respectively. Relative electrolyte leakage was found to be minimal during desiccation. Activities of antioxidant enzymes, namely peroxidase (158.33%), glutathione reductase (107.70%), catalase (92.95%) and superoxide dismutase (184.70%) were found to be higher in the desiccated state. The proline concentration increased by 1.4 fold, starch concentration decreased 3.9 fold and sucrose content increased 2.8 fold during desiccation. Upon rehydration, S. brachystachya recovered its original morphology, physiological and biochemical functions. Our results demonstrate that S. brachystachya minimizes desiccation stress through a range of morphological, physiological and biochemical mechanisms. These results provide useful insights into desiccation tolerance mechanisms for potential utilization in enhancing stress tolerance in crop plants